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1. 发明申请

WO2016201369A1 RECOMBINANT PROTEIN FIBER YARNS WITH IMPROVED PROPERTIES 审中-公开
标题翻译：具有改进性质的重组蛋白纤维纱
公开(公告)号：WO2016201369A1
公开(公告)日：2016-12-15
申请号：PCT/US2016/037084
申请日：2016-06-10
申请人： BOLT THREADS, INC. , WRAY, Lindsay , KITTLESON, Joshua
发明人： WRAY, Lindsay , KITTLESON, Joshua , BRESLAUER, David , BAINBRIDGE, Jamie, McAusland
IPC分类号： C07K14/435 , D01F4/00 , D01D5/00
CPC分类号： C07K14/435 , C07K14/00 , D01F4/00 , D02G3/02 , D02G3/042
摘要： Compositions and methods are provided for recombinant protein fiber yarns engineered to have desirable properties along with textiles made using such yarns. Recombinant protein fibers (RPFs) whose properties can be influenced by their composition, structure and processing to obtain improved combinations of mechanical properties, chemical properties, and antimicrobial properties for a given application are presented, along with methods of producing those fibers. The present disclosure also presents filament yarns, spun yarns, and blended yarns formed using these fibers that can be used to manufacture textiles suitable for different applications. Additionally, the combinations of RPFs with certain properties, and yarns and textiles produced from those yarns with certain structures yield yarns and textiles with certain properties designed for various applications.
摘要翻译：提供的组合物和方法用于与使用这种纱线制成的织物一起被设计成具有期望特性的重组蛋白质纤维纱线。提出了其特性受其组成，结构和加工影响以获得改进的机械性能，化学性质和抗微生物特性的组合的重组蛋白质纤维（RPF）以及给定应用的生产方法。本公开还提出了使用这些纤维形成的长丝纱，短纤纱和混纺纱，其可用于制造适合于不同应用的纺织品。另外，具有某些特性的RPF的组合以及由具有某些结构的纱线生产的纱线和纺织品产生具有为各种应用设计的某些特性的纱线和纺织品。

2. 发明申请

WO2018165595A1 SUPERCONTRACTING FIBER TEXTILES 审中-公开
公开(公告)号：WO2018165595A1
公开(公告)日：2018-09-13
申请号：PCT/US2018/021818
申请日：2018-03-09
申请人： BOLT THREADS, INC.
发明人： BAINBRIDGE, Jamie , WRAY, Lindsay , HEINRICH, Mitchell , KITTLESON, Joshua , BRESLAUER, David
IPC分类号： C12N15/09 , C07K14/435 , D01F6/68 , D01F4/02 , D01D5/253 , D01D5/06 , D06M13/144 , D06M23/14
CPC分类号： D06M13/144 , C07K14/43518 , D01F4/02 , D02G3/02 , D02G3/04 , D02G3/36 , D02G3/38 , D04B1/104 , D04B1/12 , D04B1/14 , D06B3/10 , D06B3/30 , D06C21/00 , D06M7/00 , D06M11/05 , D06M2101/12 , D10B2211/00 , D10B2211/04 , D10B2401/061
摘要： The present disclosure relates generally to pre-treating textiles and methods of preparing textiles in a pre-treated state. Specifically, the present disclosure relates to pre-treating textiles comprising recombinant protein fibers.

3. 发明申请

WO2018165594A1 COMPOSITIONS AND METHODS FOR PRODUCING HIGH SECRETED YIELDS OF RECOMBINANT PROTEINS 审中-公开
公开(公告)号：WO2018165594A1
公开(公告)日：2018-09-13
申请号：PCT/US2018/021817
申请日：2018-03-09
申请人： BOLT THREADS, INC.
发明人： KITTLESON, Joshua , STEVENS, Thomas , HILL, Rena , PESCE, Carlos Gustavo
IPC分类号： C07K14/00 , C07K14/435 , C40B40/02
摘要： The present disclosure relates to methods for producing recombinant proteins, as well as compositions used in and produced by such methods. Specifically, the present disclosure relates to methods for producing high secreted yields of recombinant proteins, and the compositions provided herein include recombinant host cells that comprise polynucleotide sequences encoding proteins operably linked to at least 2 distinct secretion signals.

4. 发明申请

WO2018132821A2 ELASTOMERIC PROTEINS 审中-公开
公开(公告)号：WO2018132821A2
公开(公告)日：2018-07-19
申请号：PCT/US2018/013839
申请日：2018-01-16
申请人： BOLT THREADS, INC.
发明人： KITTLESON, Joshua , LEE, Michael , BRESLAUER, David, N. , WIDMAIER, Daniel, M.
IPC分类号： C12P21/02 , C12N1/18
摘要： This invention relates to elastomeric protein and elastomeric protein production. In particular, the invention is directed to elastomeric protein sequences, including methods and compositions for production of elastomeric protein sequences, such as expression constructs, and host cells, and including compositions generated from the elastomeric protein sequences.

5. 发明申请

WO2018053204A1 LONG UNIFORM RECOMBINANT PROTEIN FIBERS 审中-公开
标题翻译：长统一重组蛋白纤维
公开(公告)号：WO2018053204A1
公开(公告)日：2018-03-22
申请号：PCT/US2017/051668
申请日：2017-09-14
申请人： BOLT THREADS, INC.
发明人： WRAY, Lindsay , PERELMAN, Loren , KITTLESON, Joshua , BRESLAUER, David
IPC分类号： C07K14/435 , D01D5/00 , D01F4/00
摘要： The present disclosure provides improved long uniform recombinant protein fibers with desirable physical traits. The present disclosure also provides compositions derived from the long uniform recombinant protein fibers.
摘要翻译：本公开提供了具有期望的物理性状的改进的长均匀重组蛋白质纤维。本公开还提供了衍生自长均匀重组蛋白纤维的组合物。

6. 发明申请

WO2017214618A1 RECOMBINANT PROTEIN FIBER YARNS WITH IMPROVED PROPERTIES 审中-公开
标题翻译：具有改进性能的重组蛋白质纤维纱
公开(公告)号：WO2017214618A1
公开(公告)日：2017-12-14
申请号：PCT/US2017/037013
申请日：2017-06-12
申请人： BOLT THREADS, INC.
发明人： BAINBRIDGE, Jamie, Mcausland , BRESLAUER, David , WRAY, Lindsay , KITTLESON, Joshua
IPC分类号： D01D5/06 , D01D5/24 , D01F6/68
摘要： Compositions and methods are provided for recombinant protein fiber yarns engineered to have desirable properties along with textiles made using such yarns. Recombinant protein fibers (RPFs) whose properties can be influenced by their composition, structure and processing to obtain improved combinations of mechanical properties, chemical properties, and antimicrobial properties for a given application are presented, along with methods of producing those fibers. The present disclosure also presents filament yarns, spun yarns, and blended yarns formed using these fibers that can be used to manufacture textiles suitable for different applications. Additionally, the combinations of RPFs with certain properties, and yarns and textiles produced from those yarns with certain structures yield yarns and textiles with certain properties designed for various applications.
摘要翻译：为重组蛋白质纤维纱线提供了组合物和方法，所述重组蛋白质纤维纱线被设计成具有期望的性能以及使用这种纱线制造的纺织品。介绍了其性质可受其组成，结构和加工影响的重组蛋白纤维（RPF），以获得针对给定应用的机械性能，化学性质和抗微生物性质的改进组合，以及生产这些纤维的方法。本公开还提供长丝纱线，短纤纱和使用这些纤维形成的混纺纱线，其可用于制造适用于不同应用的纺织品。此外，具有特定性能的RPF与由具有特定结构的纱线生产的纱线和纺织品的组合产生具有为各种应用设计的某些性能的纱线和纺织品。

7. 发明申请

WO2018165589A3 COMPOSITIONS AND METHODS FOR PRODUCING HIGH SECRETED YIELDS OF RECOMBINANT PROTEINS 审中-公开
公开(公告)号：WO2018165589A3
公开(公告)日：2018-09-13
申请号：PCT/US2018/021812
申请日：2018-03-09
申请人： BOLT THREADS, INC.
发明人： KITTLESON, Joshua , STEVENS, Thomas , HILL, Rena , PESCE, Carlos, Gustavo , BRESLAUER, David , WIDMAIER, Daniel
IPC分类号： C08H1/00 , C12P21/00 , D01F4/00
摘要： The present disclosure relates to methods for producing recombinant proteins, as well as compositions used in and produced by such methods. Specifically, the present disclosure relates to methods for producing high secreted yields of recombinant proteins, and the compositions provided herein include expression constructs, recombinant vectors, and recombinant host cells that comprise polynucleotide sequences encoding proteins operably linked to recombinant secretion signals that comprise the leader peptide of the α-mating factor (αMF) of Saccharomyces cerevisiae and a non-αMF signal peptide.

8. 发明申请

WO2018165589A2 COMPOSITIONS AND METHODS FOR PRODUCING HIGH SECRETED YIELDS OF RECOMBINANT PROTEINS 审中-公开
公开(公告)号：WO2018165589A2
公开(公告)日：2018-09-13
申请号：PCT/US2018/021812
申请日：2018-03-09
申请人： BOLT THREADS, INC.
发明人： KITTLESON, Joshua , STEVENS, Thomas , HILL, Rena , PESCE, Carlos, Gustavo , BRESLAUER, David , WIDMAIER, Daniel
IPC分类号： C07K14/435 , C07K14/395
摘要： The present disclosure relates to methods for producing recombinant proteins, as well as compositions used in and produced by such methods. Specifically, the present disclosure relates to methods for producing high secreted yields of recombinant proteins, and the compositions provided herein include expression constructs, recombinant vectors, and recombinant host cells that comprise polynucleotide sequences encoding proteins operably linked to recombinant secretion signals that comprise the leader peptide of the α-mating factor (αMF) of Saccharomyces cerevisiae and a non-αMF signal peptide.

9. 发明申请

WO2018132821A3 ELASTOMERIC PROTEINS 审中-公开
公开(公告)号：WO2018132821A3
公开(公告)日：2018-07-19
申请号：PCT/US2018/013839
申请日：2018-01-16
申请人： BOLT THREADS, INC.
发明人： KITTLESON, Joshua , LEE, Michael , BRESLAUER, David, N. , WIDMAIER, Daniel, M.
IPC分类号： C07K14/435 , C07K19/00 , C12N1/21 , C12N15/12 , C12N15/62 , C12N15/63
摘要： This invention relates to elastomeric protein and elastomeric protein production. In particular, the invention is directed to elastomeric protein sequences, including methods and compositions for production of elastomeric protein sequences, such as expression constructs, and host cells, and including compositions generated from the elastomeric protein sequences.

10. 发明申请

WO2016149414A1 IMPROVED SILK FIBERS 审中-公开
标题翻译：改进的丝光纤
公开(公告)号：WO2016149414A1
公开(公告)日：2016-09-22
申请号：PCT/US2016/022707
申请日：2016-03-16
申请人： BOLT THREADS, INC.
发明人： BRESLAUER, David , WRAY, Lindsay , KITTLESON, Joshua
IPC分类号： C12P21/02 , C07K14/435
CPC分类号： C07K14/43518 , C12P21/02 , D01D5/06 , D01D5/24 , D01D5/253 , D01F4/00 , D01F4/02 , D01F6/68
摘要： Methods and compositions are provided for improved proteinaceous block copolymer fibers based on long repeat units having molecular weight of greater than about 10 kDal. Each repeat unit includes more than about 150 amino acid residues that are organized into a number of "quasi -repeat units." The fibers have improved mechanical properties that better recapitulate those of the native silk fibers.
摘要翻译：提供了基于分子量大于约10kDa的长重复单元的改进的蛋白质嵌段共聚物纤维的方法和组合物。每个重复单元包括多于约150个氨基酸残基，其被组织成许多“准重复单位”。纤维具有改善的机械性能，更好地概括了天然丝纤维的那些。

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